Physio-chemical properties of Manikchaman variety of grapes has been measure at 79.178% (wb). Manik Chaman variety is mutants of Thompson seedless. The juice is sweet with a TSS of 20-220 B. Variety has a good keeping quality and is used for table purpose and raisin making. The hundred grams of grape contains 75 to 85 per cent moisture, 18.1 g carbohydrates, 15.48 g sugars. Size, shape and physical dimensions of grape are important in sizing, sorting and other separation process. Various physio-chemical properties of grapes has been determined i.e. Moisture content, length, width, thickness, bulk density, true density, TSS, Titrable acidity, pH, Reducing sugar, Total sugar, Non-reducing sugar, Ascorbic acid, colour yellowness index. The moisture content was 79.17%, length 29.72 mm, width 14.14 mm, thickness 17.95 mm, bulk density 1.058 g/cc, true density 1.058 g/cc, TSS 19.9°B, Titrable acidity 0.678%, pH 4.3, Reducing sugar 17.395%, Total sugar 19.417%, Non-reducing sugar 2.015, Ascorbic acid 5.88 mg, yellowness index 81.35.